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Box 2 (shipped on blue ice packs) Material not included 96-well microtiter plate Distilled or deionized water 1X PBS 10 L to 1000 L adjustable single channel micropipettes with disposable tips 50 L to 300 L adjustable multichannel micropipette with disposable tips Conical tubes and bottles for sample and buffer preparation Centrifuge and/or microfuge Sonicator or tissue homogenizer Multichannel micropipette reservoirs Ethanol Microplate reader capable of reading 405 nm Application Notes Optimal working dilution should be determined by the investigator

Gut microbiome and MASLD: a key connection Building on the understanding of MASLD and its progression to MASH, the role of the gut microbiome has emerged as a significant factor in disease pathogenesis

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Omics Technol

Following vortexing and passage through a 70 m strainer, the suspension of IECs was washed twice by centrifugation at 600 g for 3 min and collected for subsequent experiments