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The sn-2 position of PC is typically enriched with PUFAs, particularly AA, making this cleavage site especially important in cell signaling and inflammation (Lin et al., 2024)

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Briefly, RPL22Flag-expressing cells from shCtrl, shGDH1 and shGDH1 + -KG groups were treated with 100 g ml 1 CHX for 15 min before lysis and anti-Flag beads (Biomag, BMFA1000-2) were used to capture the RNARPL22 complex

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We first examined the effect of sertraline on the cell proliferation of A549 cells and found that NKX21 overexpressing A549 cells were sensitised to sertraline in serine/glycine-depleted conditions (Fig