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An actin gene was included as a reference gene (internal control)

6 , 243ra287 (2014)

Redox-state analysis DF-1 cells were lysed by freezethaw method, and reduced/oxidized glutathione (GSH/GSSG) measurement was performed using a GSH and GSSG Assay Kit (Beyotime) according to the manufacturers protocol

this indicates that there is some flexibility in nutrient usage, which is crucial to support cellular fitness (Fig

Dietary changes: B12 deficiency stems from dietary factors, like adhering to a strict vegan diet or experiencing malabsorption issues, integrating B12-rich foods into your diet proves beneficial