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Characteristics of High Affinity Ni-Charged Resin FF 1 Dynamic binding capacity conditions: Sample: 5 mg/mL (histidine) 6 -tagged pure proteins (M r 43 000) in binding buffer (capacity at 10% Breakthrough) Column volume: 1 mL Retention time: 3min Flow rate: 0.333 mL/min Binding buffer: 20 mM sodium phosphate, 500 mM NaCl, 10 mM imidazole, pH 7.4 Elution buffer: 20 mM sodium phosphate, 500 mM NaCl, 250 mM imidazole, pH 7.4 Note: Dynamic binding capacity is protein-dependent

Biberdorf RI, Spurbeck GH

An elevated glutaminolysis is related to the promotion of cancer progression at early stages by stimulating cell growth through the mTORC1 pathway and diminishing elimination of altered proteins and organelles by inhibiting autophagy (154, 162)

Curcumin activates human glutathione S-transferase P1 expression through antioxidant response element

PMID: 29688261 Bahri Khomami M, et al