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To this end, we used a high-copy plasmid genomic DNA library to perform an unbiased genome-wide suppressor screen 44 : We transformed the VMA2-AID* cells with 1588 overexpression plasmids from the library and examined the induction of V-ATPase-dependent autophagy in each transformant by using the western blot-based GFP-Atg8 processing as the readout assay

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thetaiotaomicron was isolated from C3H/HeOuJ mice fecal samples by serially diluting in 1 PBS with 0.1% L-cysteine and plating on pre-reduced brain heart infusion (BHI

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Froth at nostrils: The froth is the result of churning of air, mucous and water, by the process of respiration and is diagnostic of drowning