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Optimal ist die Kombination mit Citrullin

1 2 Essays in Biochemistry

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1) k 1 and k 2 were determined (100, 300, 1000 nM) for JNK-IN-8 on the JNK C116S surface (the values for this were 1492000 M 1 s 1 and 0.1676 s 1 compared to the results of the first measurement 1482000 M 1 s 1 and 0.15360 s 1 , respectively, 2) k 3 for irreversible JNK-IN-8 was determined based on a second experiment (1, 3, 15 nM) on the JNK WT surface and it was calculated to be 0.03181 s 1 (where the original value was 0.02952 s 1 ) (moreover, using the newly determined k 1 , k 2 a new k 3 based on the new experiment was calculated: 0.03454 s 1 ), 3) k 3 and k 4 for the reversible covalent CA-IN-8 inhibitor were determined on the JNK WT surface based on two new experiments (a: 15, 40, 70 nM