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Description

Two major pathways dominate

Ascorbate induces ten-eleven translocation (Tet) methylcytosine dioxygenase-mediated generation of 5-hydroxymethylcytosine

Breifly, the reaction consists of 1 mM 1-chloro-2,4-dinitrobenzene (CDNB), 5 mM glutathione (GSH) in 50 potassium phosphate buffer and 500 l of crude enzyme in 1 ml total volume