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To validate this hypothesis, we performed an RNAscope assay using Mobp and Mbp probes, co-stained with IHC employing GFAP and IBA1 antibodies

The same volume of PBS was intraperitoneally injected into the control mice

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Instead, a different number of active genes in the cluster has been found in other species, with rats and mice having 6 and 9 active genes, respectively [20, 21]