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Moreover, barrier dysfunction has been linked to diseases originating outside the GI tract, including IgA nephropathy (Tang et al., 2022b), MASLD (Tilg et al., 2022), and multiple sclerosis (Pellegrini et al., 2023)

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To confirm that the SAT1 flipper disruption cassettes had correctly replaced either the GDH2 or GDH3 gene after double crossover integrative transformation, the 5 and 3 junctions of disrupted alleles were PCR amplified

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