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This coordinated response ensures a multi-layered chemical defense, where flavonoids quench infection-induced ROS and reinforce cell walls, while anthraquinones act as direct antimicrobial agents targeting membrane integrity or respiratory enzymes

The expression levels of GPX4 were determined by Western blotting, measuring the grayscale ratios of the GPX4 band to the GAPDH band in each group, with the ratio in the Control group normalized to 1

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One of the most important risk factors for glaucoma is elevated intraocular pressure (IOP), which promotes mechanical stress in RGCs and the lamina cribrosa, blockading axonal transport and causing axonal degeneration 3,4