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The reassembly (&/or one or more additional directed evolution methods described herein) and screening methods of the invention can be used to optimize the antigen structure (and sequence) for subsequent processing after uptake by APC so that intracellular processing results in derivation of the required peptide fragments for presentation on Class I or Class II on APC and desired immune responses

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In recent years, significant research has explored the use of probes in medical biology, particularly for acute kidney injury (AKI), a condition with high incidence and mortality rates