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For negative runs, buffers consisted of 0.03% ammonium hydroxide and 5% methanol in water for mobile phase A, and, for mobile phase B, 0.03% ammonium hydroxide, 5% water, and 35% methanol in isopropanol

The resulting oxidative stress not only impairs hair growth but can also accelerate the aging process, leading to premature graying and overall hair quality deterioration