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Furthermore, we observed an enhanced degradation of nucleotide bases, as reflected in lower levels of xanthosine 5-triphosphate and subsequent accumulation of purine (i.e., hypoxanthine, uric acid, 5-hydroxyisouric acid, allantoin) and pyrimidine (i.e., ureidosuccinic acid, N-acetylcytidine) catabolites

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After the final wash, the pellet was resuspended in 40 l of 1x electrophoresis sample buffer (40% glycerol, 240 mM Tris/HCl pH 6.8, 8% SDS, 0.04% bromophenol blue, 5% -mercaptoethanol) and analyzed by SDS-PAGE western blot

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