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The concentration of denaturant used for pellet washing is determined empirically and should be below the concentration required for solubilization of the recombinant protein

[DOI] [PubMed] [Google Scholar] 51.Maslowski KM, Vieira AT, Ng A, Kranich J, Sierro F, Yu D, Schilter HC, Rolph MS, Mackay F, Artis D, Xavier RJ, Teixeira MM, Mackay CR

In the B16F10 model, for CD45 + cells, Fgf2 mRNA levels were significantly upregulated, and Fgfr2 mRNA levels were decreased in the VEGFR2-Fcexpressing tumors compared with in the Mock tumors, although the level changes were small (Fig

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