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Journal of Molecular Neuroscience, 72(6), 13001312

10.1016/j.joca.2021.12.007 97 HuangH.LouZ.ZhengS.WuJ.YaoQ.ChenR.et al (2022b)

Additionally, PCLS should be combined with advanced imaging techniques to visualize three-dimensional cell-cell interactions, overcoming the limitations of two-dimensional imaging

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The mixture is then passed through a fresh glutathione resin column to remove the cleaved GST tag, allowing the purified target protein to be collected in the flow-through