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A multicenter randomized controlled study of Xinsuning capsules in the treatment of phlegm-heat disturbed heart syndrome with premature ventricular contractions

For fluorescence measurements, 20 l samples were removed at distinct time points and mixed with 180 l of a 55.6 M solution of Thioflavin T (ThT) (SigmaAldrich) in 0.5 PBS and analyzed in a FluoroMax-3 spectrofluorometer (HORIBA Jobin Yvon, Bensheim, Germany) using an excitation wavelength of 450 nm and an emission wavelength of 482 nm

Finally, the eluted samples were concentrated and analyzed using SDS-PAGE and UV-vis spectroscopy

doi: 10.1038/s42255-018-0009-4