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The PCR parameters were as follows: A denaturation step of 98C for 30 seconds, followed by 30 cycles of a melting step at 98C for 10 seconds, an annealing step of 55C for 20 seconds, an extension step of 72C for 25 seconds, followed by a final extension step at 72C for 5 minutes

Weather conditions Temperature and humidity are known technical confounders when measuring nicotine and 3-EP 15

Dimensions: 6500 x 3497 px | 55 x 29.6 cm | 21.7 x 11.7 inches | 300dpi Date taken: 9 March 2023 More information: This image could have imperfections as its either historical or reportage

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