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Evening administration is not recommended

So 12 ml of whole blood concentrated down to 3 ml of PRP would be 4X or four times more concentrated platelets in the PRP than in the whole blood

Gottfries, CG (1997)

For this example, we'll stick with 2 mL

Those with naturally high NNMT activity might respond more dramatically to inhibition, while those with lower baseline activity might see more modest effects