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After being washed with HBSS, the cells were observed immediately with a fluorescence microscope

These mechanisms include enhanced metabolic activity of drug-detoxifying proteins such as glutathione-S-transferase, increased efflux of the drug partially caused by upregulation of ATP-binding cassette (ABC) transporters, changes in microtubule formation and kinetics due to overexpression of III-tubulin, and mutations in tumor suppressor genes [101]