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Analytes (M-CuP or D-CuP) were diluted in PBST buffer (10 mM phosphate-buffered saline, 0.05% Tween-20, pH 7.4) and injected at increasing concentrations at a flow rate of 30 L/min, with 120 s association and 180 s dissociation phases

Repeated-measure ANOVA was used to determine statistically significant changes between groups over time

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What community sources describe: Self-reported community timelines describe months one to two as the period when weight change becomes clearly visible rather than just felt as reduced appetite

Biologically, SARSCoV2 can invade the central nervous system, potentially disrupting the suprachiasmatic nucleus and triggering neuroinflammation that destabilizes sleepregulatory centers ( 4.4.3 Obstructive sleep apnea as a plausible underlying mechanism The hallmark of Class 3long time in bed coupled with poor subjective sleep qualityalso raises the possibility of undiagnosed obstructive sleep apnea syndrome (OSAS)