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In the challenge test, in accordance with the assumptions of the PN-EN ISO 11930:2019 standard, nephelometric-calibrated suspensions of test microorganisms (N) with a density in the range of 1 10 7 1 10 8 unit/ml for bacteria and 1 10 6 1 10 7 unit/ml for fungi were used to contaminate the test samples

Br J Nutr 110(8):13571368

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To confirm these observations, purified PDI was treated with different concentrations of 4-HNE in vivo (20200 M) to show a 1456% inhibition, respectively

This phenomenon, known as metabolic endotoxemia, has been linked to: Chronic inflammation Insulin resistance Obesity Metabolic syndrome In animal studies, even modest increases in circulating endotoxins have been shown to trigger inflammatory responses that interfere with metabolic regulation