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Changes in lipid and lipoprotein metabolism were not detected in cardiac MTs using DEGs, but similar effects of DMSO were observed for citric acid cycle and respiratory electron transport (q-value: 1.3 * 10 12 , 58 DEGs out of 171 genes, 65.5% downregulated) and glucose metabolism (q-value: 2.8 * 10 3 , 20 DEGs out of 77 genes, 55.0% downregulated), though less genes were downregulated in cardiac MTs compared to hepatic MTs

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To validate our refinement protocol, we used phenix.mtriage 50 to calculate FSC curves of our models against the full map and half maps 53 (Supplementary Fig