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doi: 10.3389/fimmu.2023.1282890 Received 29 August 2023 Accepted 26 October 2023 Published 20 November 2023 Volume 14 - 2023 Edited by Ying Ying Leung, Singapore General Hospital, Singapore Reviewed by Jie Lu, The Affiliated Hospital of Qingdao University, China

Furthermore, a novel replication origin and antibiotic resistance marker (spectinomycin) allowed an orthogonal use in combination with pEVOL

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However, for concentrations exceeding three g/dl, dilution with a 0.1 M phosphate buffer at pH 7.0 was necessary to obtain reliable results