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17 ) using the primary antibodies (see Supplemental Table 4) against III-Tubulin, microtubule associated protein 2 (MAP2), tyrosine hydroxylase (TH), FOXA2, and PAX6 followed by the secondary antibody AF 488 donkey anti-mouse (A21202, Invitrogen) and Cy3 donkey anti-mouse (715-165-150, Jackson)

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