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A specific mass of Brusatol will be weighed and dissolved in DMSO to prepare a stock solution of 25.0 mg/ml, which will be aliquoted and stored at 20 C for future use.The calculation of the specific dosing regimen is as follows: The actual body weight of the mice will be measured, and the working solution will be prepared according to the recommended solubilization scheme in the product manual: 10% DMSO (from the stock solution) 40% PEG300 5% Tween-80 45% sodium chloride

Taken together, these data demonstrate that PTEN, a negative regulator of Akt signaling, can decrease sensitivity to glucose withdrawalinduced TK induction and cell death

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