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Briefly, GSTO1-1 pQE30 plasmid was transformed into M15 cells, which were grown in Luria-Bertani broth supplemented with 100 g ml 1 ampicillin and 25 g ml 1 kanamycin at 37 C to an OD 600 of 0.8 and induced with 0.1 mM isopropyl thio- -D-galactoside for 4 h

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The total run time per sample was 20 min

Identification of a tumor-promoter cholesterol metabolite in human breast cancers acting through the glucocorticoid receptor

Currently, clinical studies evaluating the therapeutic value of statins reducing the inflammatory exacerbations observed in severe COVID19 patients are ongoing (ClinicalTrials.gov Identifier: NCT04407273, NCT04472611, NCT04348695, NCT04380402)