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Finally, Western blot and qPCR analysis were performed in liver biopsies of twelve obese patients following bariatric surgery with NAFLD activity scores (NAS) ranging from 0 to 3, and a microarray analysis of ACLF patients was examined

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Briefly, 25-30 million cells were collected and washed twice with cold saline (0.9% NaCl), scraped into 1 ml of cold KPBS, and pelleted via centrifugation at 1,000g for 1.5min at 4C

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