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Plasmids construction Clasp2-WT encoding mouse WT Clasp2 was subcloned into EcoRI and SalI sites of the pEGFP-C1 vector to construct the pEGFP-C1-Clasp2-WT plasmid, which was further used as a template to construct pEGFP-C1-Clasp2-S1025A and pEGFP-C1-Clasp2-S1025D by site-directed mutagenesis

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To perform its protective functions, GSH depends on nicotinamide adenine dinucleotide phosphate (NADPH) produced through the pentose phosphate pathway