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for 10 min and the following for 40 cycles: 95 C

Ballestri, S

Figure 6 The TrM is dispensable for the interaction of HpaA with components of the T3S system In addition to GST pull-down assays and co-immuno- precipitation experiments, we performed bacterial two hybrid-based interaction studies to confirm the interaction of HpaA with T3S system components and to further investigate the contribution of the TrM to the identified interactions