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To examine whether NAC (pharmacological antioxidant, Sigma-Aldrich) recovered MS-induced T-cell hyporesponsiveness, we pretreated MS (0.4 mM) for 48 h in the absence or presence of 5 mM NAC, then treated Con A (5 g/ml) for 8 h in Jurkat T cells

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Wash your hands for twenty seconds before handling and clean utensils well to prevent cross contamination