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Through this, the patients health history, diet, and other related details may be gathered and factored in

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Moreover, it was shown experimentally that Gch1 (gene encoding the rate-limiting enzyme of BH4 biosynthesis) is a direct target of Nrf-2 in skin cells submitted to radiation (Nrf-2 has a binding site in the proximal promoter of the Gch1 gene), and the resultant increased intracellular BH4 levels promoted cytoprotection by neutralizing ROS [108]

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The cells were washed twice in 1 mL ice-cold PBS and lysed with 5 pellet volume of RIPA buffer (20 mM HEPES, 100 mM NaCl, 0.1% Triton-X, 0.2% SDS, PhosStop, cOmplete inhibitor tablet)