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Time-dependent responses have also been observed in juvenile seabream, where significant MT induction occurred 12, 24, and 48 hours after exposure to CdCl 2 , indicating that the timing of sampling is crucial for accurately capturing MT expression patterns following heavy metal challenge ( znt1, znt5, and znt7 ), Zn-regulated iron-regulated transporter-like proteins ( zip8 and zip10 ), and MTs