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After incubation for 10 min, 200 L luciferin solution (containing 20 mM tricine, 1.0 mM MgSO4, 0.1 mM EDTA, 33 mM dithiothreitol, 270 M coenzyme A, 500 M D-luciferin, and 530 M ATP, pH 7.8) was added, and then the firefly luminescence was measured with a BioTek synergy 4 Multi-Mode Microplate Reader

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T., Noda, M., O'Regan, A

Glutamate induced the formation of peroxynitrite, mainly localized within the mitochondria, and triggered delayed (6 h) calcium overload, mitochondrial depolarization, lipid peroxidation, and massive neuronal cell death

TB-500 appears to function mainly through actin-binding and subsequent cellular organization