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2.5 Potential biomarkers of ferroptosis Translating ferroptosis from a mechanistic concept into a clinical tool for IPF will require reliable, minimally invasive biomarkers that reflect iron overload, lipid peroxidation, and antioxidant failure in the lung

We used metformin treatment to induce aging-like metabolic stress in human primary fibroblasts 14 , and combined metformin exposure with Pol I inhibition by the compounds BMH-21 and CX-5461 32,33 , with direct ATP repletion serving as a positive control

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Oxidative stress releases pro-inflammatory molecules that interact with ROS to produce inflammation, performing major role in early onset of aging and metabolic diseases [7]