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HC El Greco: Jos Enrique Mario Surez, Jos Luis Quintana Gmez, Jos Antonio Gonzlez-Posada Delgado, Enrique Revilla Pascual, Esperanza Duralde Rodrguez, Milagros Beamud Lagos

Insulin receptors are a class of special protein heterodimers, composed of a calcitonin receptor (CTR) combined with three homologous receptor activity-modifying proteins (RAMP 1,2,3), forming three subtypes (AMY1, AMY2, AMY3)

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For immunofluorescence, primary antibodies were incubated overnight at 4 C after treatment with TRITC-, CY3-, or CoraLite488-conjugated goat secondary antibodies (Proteintech, China)