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Many people are cutting their breasts off because they have this gene, which doesn't always mean you're going to get breast cancer

10.1016/j.tig.2008.05.006 Trends Genet

Abdominal aorta was isolated and digested for 1 h, 37 C, 45 rpm agitation in HBSS with 450U/ml Collagenase I (Sigma), 125U/ml Collagenase XI (Sigma) 60U/ml Hyalurase (Sigma), 60U/ml DNAse1 (ThermoFisher) and filtered through 100 m cell strainers (Sysmex) to achieve a single cell suspension