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Discussion Long-term clinical experience demonstrates the high frequency of failure in the treatment protocols followed in pediatric and geriatric patients

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A single-cell RNA-Seq (scRNA-Seq) study analyzed primary NP and AF cells isolated from healthy adult discs and observed that receptor signaling pathway genes were highly expressed in the AF, whereas protein synthesis and ECM genes were highly expressed in the NP (Fernandes et al., 2020)

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In addition, these protein-DNSC conjugates are sensitive to theirimmediate environment