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It had a wide lumen with hyalinized center or contained few numbers of sperms (Fig

Graphical abstract Table 1

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10.1056/NEJMoa2031054 184

Organoid preparation and culture To generate gastric cancer organoids, fresh tumor tissues were transported on ice in cold DMEM/F12 (Gibco) with primocin (InvivoGen), then washed in cold PBS, minced into fragments (1 mm), and digested for 1 h at 37 C with collagenase and hyaluronidase