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Mice from each of the age groups were utilized within an experimental period

high copper intake downregulates intestinal absorption and upregulates biliary excretion, whereas deficiency states promote intestinal uptake and reduce biliary loss to conserve copper [89]

These findings should be further validated in cancer patients undergoing radiation, as the available data are insufficient to support them

After 30 minutes at 37C, 500 L of cold ethanol was added to the homogenate to precipitate proteins, followed by centrifugation (21,000 g , 4C, 10 minutes), and the supernatant was dried under an N 2 stream at room temperature