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These were allowed to adhere overnight in full culture media (DMEM (41965039) with 4 mM L-glutamine, 10% dFBS (26400044), 1 mM sodium pyruvate (11360039) and 50 U ml 1 penicillinstreptomycin (15070063)) under normal cell culture conditions

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The gut microbiome is also essential for serotonin production, and gut microbes acting through short-chain fatty acids have been shown to modulate serotonin production in preclinical and in vitro human models [86]

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