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To each yeast cell pellet, 300 L of ice-cold extraction solvent with internal standard were added to each sample, followed by immediate probe sonication for 20 s at power level 2, 20% duty cycle using a Branson 450 probe sonicator

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The formulations also were posited to be impactful when tested alongside agents that may be associated with alopecia

Patients and staff should be warned or be aware of this potential cause of self-limiting urine discolouration to avoid unnecessary anxiety and investigation