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For G protein and -arrestin2 receptor recruitment studies, we used human G i1 with nanoluciferase (NanoLuc) inserted at position 91 (NanoLuc-G i1(91) ) and human -arrestin2 fused with NanoLuc at the N terminus (NanoLuc--arrestin2)

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Regarding characteristics related to rapid eye movement sleep, there was no discernible impact (Aslan et al

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91, S140S144

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