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The culture media was substituted by those including DMSO or ZIL and the cells were cultured until the control sample (which does not contain the solvents) became 80% confluent

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2.2.6 Other functions 20% of GM commensal species in mice and 10% of GM in humans exhibit circadian oscillations, GM regulates circadian rhythms of transcripts in a variety of tissues in the periphery and in the brain, and GM imbalance can lead to sleep disorders triggering psychiatric disorders [30]