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1b), and an analysis across the entire cohort showed an overall improvement in ppFEV 1 from a median (first quartile, third quartile) 36 (23, 49), or mean 37 5.5 s.e.m., before therapy to a median 42 (27, 67), or mean 45 6.9 s.e.m., after therapy, which is median or mean ppFEV1 difference of 6 and 8, respectively ( P = 0.004, Wilcoxon signed-rank two-tailed t -test)

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The optimizations and full runs of the multiplex panel were executed using the seqIF methodology integrated into the Lunaphore COMET platform (characterization 2 and 3 protocols, and seqIF protocols, respectively) 97