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The Caco-2 monolayer (32) is a standard in vitro intestinal model, but it lacks a mucus layer and its tight junctions resemble those of the colon, with narrower pores (4.5 ) (33, 34) compared to the small intestine (89 ) (35) in a polarized monolayer

A: Ampoules should be stored in a cool, dry, and dark place below 25C, away from direct sunlight, to maintain potency and safety throughout their 24-month shelf life

Cerebellar organotypic cultures Culture preparation Pups from newborn (P0) RjOrl:SWISS mice (Janvier) were euthanized by decapitation, and cerebellar organotypic cultures were prepared as previously described 115

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Unreconstituted lyophilised vials should be stored according to the manufacturer's or sponsor's instructions