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To overcome this limitation, Lohman and Remington (2008) further engineered roGFP1 by replacing the chromophore-interacting H148 by serine and inserting an additional amino acid between the disulfide forming C147 and S148

Mitochondrial diseases are characterized by both a diminished capacity to produce ATP as well as an elevated production of reactive oxygen species (ROS) and reactive nitrogen species (RNS) [3]

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Pregnant and nursing women known to be heterozygous for the deficiency also should avoid ingestion of drugs with oxidant potential, because some gain access to the fetal circulation and to breast milk

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