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The calpain inhibitor, calpastatin, has been shown to attenuate Mn-induced toxicity by reducing proinflammatory markers, including IL-1, TNF-, NF-B in the rat hippocampus and striatum, preventing Mn-induced motor deficit and microglial Iba1 expression and dopamine dysregulation in rat striatum ( Melatonin, which has been shown to afford neuroprotection via its anti-inflammatory and antioxidant properties in PD and AD models (Park and Chun, 2017b)

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48 h after transfection, cells were washed twice with pre-warmed PBS and fixed with 4% paraformaldehyde (PFA) for 1015 min

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