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figures are shown for both 5 days/week and 3/week patterns

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Lactate Dehydrogenase (LDH) Assay The relative amounts of LDH released into the media (conducted in triplicates for each group) were determined using CytoTox-ONE Homogeneous Membrane Integrity Assay kit Cat.#G7892 (Promega, USA) according to the manufacturers instructions

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These responses are often attributed to concentration variables or tissue-level signaling effects