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290,291,292 Accumulating macrophages release cytokines and chemokines, including IL-1, IL-6, chemokine ligand 2 (CCL2), and TNF-, intensifying the inflammatory environment and aggravating nerve damage

In contrast, in IFN-R / mice cuprizone-induced microglia accumulation was initially diminished or simply delayed (Mana et al., 2006) that was reflected at week 3 of cuprizone diet by a reduced expression of microglia-associated molecules such as IGF-1 and TNF- that have been proposed to promote proliferation of OPC

Age influences microglial activation after cuprizone-induced demyelination