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Methods: Non-alcoholic steatohepatitis (NASH)-HCC model: C57BL/6 and DKK3 knockout (DKK3 -/- ) mice were fed with choline-deficient high fat diet for 12 months to induce NASH and NASH-HCC

10.1111/jfbc.13316 63 OwumiS

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and (4) conducting well-designed RCTs with validated AMPK activity markers (e.g., p-AMPK Thr172 in peripheral blood mononuclear cells or liver tissue) to establish causal links between TCM-mediated AMPK activation and clinical outcomes